产品名称：缓冲MUG琼脂
Product name：Buffered MUG Agar
产品用途：用于滤膜法中MUG底物检测食品中大肠杆菌。
Product usage: Used to detect Escherichia coli in food by MUG substrate in filter membrane method.
配方成分
Formula ingredients
|
配方 Formula |
含量（g/L） Content (g/L) |
|
磷酸氢二钠 Disodium hydrogen phosphate |
8.23 |
|
磷酸二氢钠 Sodium dihydrogen phosphate |
1.20 |
|
4-甲基伞形酮-β-D葡萄糖苷酸(MUG) 4-methylumbellione-β-D glucuronide (MUG) |
0.1 |
|
氯化钠 Sodium chloride |
5.0 |
|
琼脂 Agar |
15.0 |
|
PH |
7.2-7.6 25℃ |
使用方法
Usage
称取本品29.5g，加热煮沸完全溶解于1000ml蒸馏水中，分装，121℃高压灭菌15分钟，冷至45-50℃时，倾入无菌平皿，备用。
Weigh 29.5g of this product, heat and boil completely dissolved in 1000ml distilled water, divided, 121 ℃ autoclave sterilization for 15 minutes, cold to 45-50 ℃, poured into a sterile flat dish, standby.
